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For centuries, plants have played a vital role in medicinal practices. Flavonoids, polyphenols, and anthocyanins found in plants provide important antioxidant benefits. Aristotelia chilensis (Maqui) and Berberis microphylla (Calafate) are a tree and an evergreen shrub native to the temperate rainforests of Chile and southern Argentina and are both recognized for their antioxidant-rich berries. Neuroblastoma (NB) is a tumor of the peripheral nervous system and is the most common cancer and extracranial solid tumor in infants. N18TG2 is an established mouse NB cell line. We hypothesize that Maqui leaves extract and Calafate berry extracts exert anti-proliferative effects on N18TG2 cells.
A tablet that effectively combines immediate and modified-release properties will be developed by compressing the tablet with a hollow core, applying an enteric coating, and filling the core with concentrated drug granules. Acetaminophen will be used as a model drug. The thickness, hardness, friability, and disintegration tests will be performed on the tablets. Key manufacturing parameters, including formulation matrix, choice of excipients, and coating will be optimized to achieve the desired release profile. A design of experiment (DoE) approach will be implemented via to systematically evaluate and refine these parameters, ensuring the effective release and stability of the formulation.
The study aims to express and purify the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein using recombinant protein techniques and evaluate its binding affinity to the human ACE2 receptor. HEK293T cells were transfected with two plasmids: one expressed RBD fused with a His-tag allowing protein purification via immobilized metal affinity chromatography (IMAC) and the other expressed RBD fused with an sfGFP-tag allowing fluorescence detection of protein expression. Purification of the recombinant His-tagged RDB and sfGFP-tagged RBD protein products using IMAC, Hydrophobic interaction chromatography (HIC) and Ion exchange chromatography is ongoing. BCA protein assay, SDS-PAGE, Western blotting, and ELISA will be performed to assess expression yield, purity, and binding interactions. The research enhances understanding of the effects of two common tags on SARS-CoV-2 RBD dynamics and their potential applications in diagnostics, therapeutics, and vaccine development against COVID-19.
